Issue |
Ciência Téc. Vitiv.
Volume 38, Number 1, 2023
|
|
---|---|---|
Page(s) | 35 - 42 | |
DOI | https://doi.org/10.1051/ctv/ctv20233801035 | |
Published online | 07 March 2023 |
Article
Effect of drought on aquaporin expression in grafted and ungrafted grapevine cultivars
Efeito da seca na expressão da aquaporina em cultivares de videira enxertadas e não enxertadas
1
Kilis 7 Aralik University, Department of Herbal and Animal Production, Vocational School of Technical Sciences, Kilis, Turkey
2
Gaziosmanpasa University, Department of Horticulture, Tokat, Turkey
* Corresponding author: Tel.: + 905077633352; e-mail: mk_mehmetkoc@outlook.com
Received:
14
October
2022
Accepted:
3
February
2023
Drought stress severely affects growth, development and productivity in most agricultural crops. Since ancient times, rootstocks have been used to enable crop cultivation in unsuitable soil conditions. In the present study, three factors were evaluated: 1) cultivar: Vitis vinifera L. cv. ‘Horozkarası’ (drought-tolerant) and cv. ‘Kabarcık’ (drought-sensitive) were used; 2) rootstock: each cultivar was self-rooted and grafted onto ‘Rupestris du Lot’ rootstock; 3) drought stress: half of each cultivar/rootstock combination underwent drought stress and the other half was irrigated at field capacity for seven days. In order to estimate the responses of the cultivars, relative water content, proline content and aquaporin isoform expression levels (VvPIP2;1, VvPIP2;2, VvTIP1;1, and VvTIP2;1) were quantified. The results revealed that drought stress caused more reduction in relative water content (RWC) in ‘Kabarcık’ cultivar (drought-sensitive) than in ‘Horozkarası’ cultivar (drought-tolerant). Proline content increased in both cultivars in response to drought stress but to a relatively greater extent in the grafted ‘Kabarcık’ cultivar. Considering expression levels of genes, VvPIP2;1, VvPIP2;2, and VvTIP2;1 were downregulated whilst VvTIP1;1 was upregulated in the leaf. Both ‘Horozkarası’ and ‘Kabarcık’ cultivars showed similar trends in terms of their responses to drought stress. Grafting significantly increased the proline content in both cultivars exposed to drought stress. The rootstock conferred better drought protection to ‘Kabarcık’ cultivar than to ‘Horozkarası’ cultivar.
Resumo
O stress hídrico afeta consideravelmente o crescimento, o desenvolvimento e a produtividade na maioria das culturas agrícolas. Desde a antiguidade, os porta-enxertos têm sido utilizados para permitir as culturas em condições de solo inadequadas. No presente estudo, foram avaliados três fatores: 1) cultivar: foram utilizadas as cultivares ‘Horozkarası’ (tolerante à seca) e ‘Kabarcık’ (sensível à seca) da espécie Vitis vinifera L; 2) porta-enxertos: cada cultivar foi auto-enraizada e enxertada no porta-enxertos ‘Rupestris du Lot’; 3) stress hídrico: metade de cada combinação cultivar/porta-enxertos foi sujeita a stress hídrico e a outra metade foi irrigada à capacidade de campo durante sete dias. Para estimar as respostas das cultivares, procedeu-se à quantificação do teor relativo de água, do teor de prolina e dos níveis de expressão da isoforma aquaporina (VvPIP2;1, VvPIP2;2, VvTIP1;1 e VvTIP2;1). Os resultados revelaram que o stress hídrico causou maior redução no conteúdo relativo de água (RWC na cultivar ‘Kabarcık’ (sensível à seca) do que na cultivar ‘Horozkarası’ (tolerante à seca). O teor de prolina aumentou em ambas as cultivares em resposta ao stress hídrico, mas em maior quantidade na cultivar enxertada ‘Kabarcık’. Em relação aos níveis de expressão dos genes, foram regulados negativamente os genes VvPIP2;1, VvPIP2;2 e VvTIP2;1 enquanto o gene VvTIP1;1 foi regulado positivamente na folha. Tanto a cultivar ‘Horozkarası’ como a cultivar ‘Kabarcık’ mostraram tendências semelhantes no respeitante às suas respostas ao stress hídrico. A enxertia provocou um aumento significativo do teor de prolina em ambas as cultivares sujeitas ao déficit hídrico. O porta-enxertos conferiu melhor proteção contra a seca na cultivar ‘Kabarcık’ do que na cultivar ‘Horozkarası’.
Key words: Horozkarası / Kabarcık / drought-tolerant / rootstock / gene expression
Palavras-chave: Horozkarası / Kabarcık / resistência à seca / porta-enxertos / expressão genética
© Koc et al., 2023
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