Issue |
Ciência Téc. Vitiv.
Volume 34, Number 1, 2019
|
|
---|---|---|
Page(s) | 15 - 24 | |
DOI | https://doi.org/10.1051/ctv/20193401015 | |
Published online | 19 April 2019 |
Determination of downy and powdery mildew resistance of some Vitis spp.
Determinação da resistência ao míldio e oídio de algumas Vitis spp.
1
Ankara University Faculty of Agriculture Department of Horticulture Diskapi-Ankara, Turkey
2
Atatürk Horticulture Central Research Institute, Viticulture ; Yalova, Turkey
* corresponding author: Murat Akkurt, Tel: +90 530 5601252, e mail: akkurt@agri.ankara.edu.tr
Received:
10
May
2018
Accepted:
9
April
2019
The Black Sea region of Turkey receives an annual rainfall of 2000-2500 mm and viticulture in the region depends on the rainfall limits significantly. In this study, the resistance of eighty different Vitis species and cultivars/genotypes to downy and powdery mildew was assessed using Marker Assisted Selection coupled with inoculation observations. Six Simple Sequence Repeats (SSR) and one Sequence Characterized Amplified Region (SCAR) markers were developed for different resistance loci (Run1, Rpv1, Ren1, Rpv3, Ren3) from different Vitis genetic resources. Eight of the cultivars/genotypes were characterised as resistant to downy mildew, seven were resistant to powdery mildew, and four were resistant to both diseases. These results, taken together with the disease inoculation observations, revealed ‘Köfteci Üzümü’ (Vitis labrusca) and ‘Giresun 3’ (Vitis labrusca) were the most resistant cultivars/genotypes.
Resumo
A região do Mar Negro na Turquia apresenta uma precipitação anual de 2000-2500 mm, sendo a viticultura na região muito limitada pela precipitação. Neste estudo, a resistência ao míldio e oídio foi avaliada em oitenta diferentes espécies e cultivares/genótipos de Vitis através da Seleção Assistida por marcadores moleculares, juntamente com observações de inoculação no fenótipo. Foram usados seis microssatélites (SSR) e um marcador de Região Amplificada Caracterizada por Seqüência (SCAR) desenvolvidos para diferentes loci de resistência (Run1, Rpv1, Ren1, Rpv3, Ren3), a partir de diferentes recursos genéticos de Vitis. Oito cultivares / genótipos foram caracterizadas como resistentes ao oídio, sete ao míldio e quatro a ambas as doenças. Estes resultados avaliados em conjunto com as observações de inoculação da doença, revelaram que os cultivares/genótipos ‘Köfteci Üzümü’ (Vitis labrusca) e ‘Giresun 3’ (Vitis labrusca) foram os mais resistentes.
Key words: Fungal Diseases / Grapevine / Resistant Loci / Inoculation
Palavras-chave: Doenças fúngicas / Videira / Loci resistentes / Inoculação
© Instituto Nacional de Investigação Agrária e Veterinária, I.P. / Ex-Estação Vitivinícola Nacional 2019
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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